assays human fstl1 elisa kits r d systems dy1694 mouse fstl1 elisa kits  (R&D Systems)

Journal: The Journal of Craniofacial Surgery

Article Title: FSTL1 Can Be a Promising Target in TMJ Osteoarthritis via Regulating Chondrocyte Mitophagy and Apoptosis

doi: 10.1097/SCS.0000000000010906

Figure Lengend Snippet: (A) Typical CBCT scan of TMD patients without OA. The red arrow showed the relatively normal bone margins. (B) Typical CBCT scan of TMD patients with OA. The red arrow showed the bone resorption in condyle. (C) The ELISA results of FSTL1 in TMJ auricular synovium fluid. (D) The ROC curve of the diagnosis value FSTL1 concentration in TMJ OA progress. CBCT indicates cone beam computed tomography; FSTL1, follistatin-like protein 1; OA, osteoarthritis; ROC, receiver operating characteristic; TMD, temporomandibular joint disorder; TMJ, temporomandibular joint.

Article Snippet: FSTL1 concentration in synovium fluid was measured by Human FSTL1 ELISA Kit (Cusabio) according to the kit instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Concentration Assay, Computed Tomography

Journal: The Journal of Craniofacial Surgery

Article Title: FSTL1 Can Be a Promising Target in TMJ Osteoarthritis via Regulating Chondrocyte Mitophagy and Apoptosis

doi: 10.1097/SCS.0000000000010906

Figure Lengend Snippet: (A) The Rt-qPCR results of FSTL1 with the targeting plasmid transfection. (B) The western blot results of FSTL1 with the targeting plasmid transfection. (C) The Rt-qPCR results of the mRNA fold change of related typical markers to chondrocyte synthesis and catabolism as well as under the inflammatory stimulation. (D) The western blot results of changes of related typical markers to chondrocyte synthesis and catabolism as well as under the inflammatory stimulation. (E) The safranine O staining of chondrogenic cells with / without FSTL1 overexpression. FSTL1 indicates follistatin-like protein 1; Rt-qPCR, quantitative reverse transcription polymerase chain reaction.

Article Snippet: FSTL1 concentration in synovium fluid was measured by Human FSTL1 ELISA Kit (Cusabio) according to the kit instructions.

Techniques: Quantitative RT-PCR, Plasmid Preparation, Transfection, Western Blot, Staining, Over Expression, Reverse Transcription, Polymerase Chain Reaction

Journal: The Journal of Craniofacial Surgery

Article Title: FSTL1 Can Be a Promising Target in TMJ Osteoarthritis via Regulating Chondrocyte Mitophagy and Apoptosis

doi: 10.1097/SCS.0000000000010906

Figure Lengend Snippet: (A, B) The mitosox probe staining and the quantified analysis of the mitochondrial morphology and function with / without FSTL1 overexpression. (C, D) The western blot and the quantified analysis results of mitochondrial function with / without FSTL1 overexpression and inflammatory stimulation. (E, F) Flow cytometry analysis of the chondrocytes apoptosis rate with / without FSTL1 overexpression. (G, H) Flow cytometry analysis of the chondrocytes cell cycle with / without FSTL1 overexpression. FSTL1 indicates follistatin-like protein 1.

Article Snippet: FSTL1 concentration in synovium fluid was measured by Human FSTL1 ELISA Kit (Cusabio) according to the kit instructions.

Techniques: Staining, Over Expression, Western Blot, Flow Cytometry

Journal: The Journal of Craniofacial Surgery

Article Title: FSTL1 Can Be a Promising Target in TMJ Osteoarthritis via Regulating Chondrocyte Mitophagy and Apoptosis

doi: 10.1097/SCS.0000000000010906

Figure Lengend Snippet: (A) The Rt-qPCR results mRNA fold change with the targeted si-FSTL1. (B) The western blot results of FSTL1 with the targeting si-RNA and its impact of typical markers to chondrocyte synthesis and catabolism. (C, D) The mitosox probe and IF staining of mt-COⅡ and the quantified analysis of the mitochondrial morphology and function with / without downregulation of FSTL1 under inflammatory stimulation. (E, F) The western blot and the quantified analysis results of mitochondrial function with / without downregulation of FSTL1 and inflammatory stimulation. (G, H) Flow cytometry analysis of the chondrocytes apoptosis rate with / without FSTL1 downregulation. FSTL1 indicates follistatin-like protein 1; Rt-qPCR, quantitative reverse transcription polymerase chain reaction

Article Snippet: FSTL1 concentration in synovium fluid was measured by Human FSTL1 ELISA Kit (Cusabio) according to the kit instructions.

Techniques: Quantitative RT-PCR, Western Blot, Staining, Flow Cytometry, Reverse Transcription, Polymerase Chain Reaction

Journal: The Journal of Craniofacial Surgery

Article Title: FSTL1 Can Be a Promising Target in TMJ Osteoarthritis via Regulating Chondrocyte Mitophagy and Apoptosis

doi: 10.1097/SCS.0000000000010906

Figure Lengend Snippet: (A, B) HE and safranin O/Fast green staining of the cartilage in the sham group and UAC-induced OA rats with si-NC or si-FSTL1 injected group. Scale bar=100 μm. (C) IHC staining of the cartilage in the sham group and UAC-induced OA rats with si-NC or si-FSTL1 injected group. Scale bar=100 μm in the larger field of view and 50 μm in the smaller field of view. (D) The gross OARSI score of the cartilage condition in the sham group and UAC-induced OA rats with si-NC or si-FSTL1 injected group. (E) The statistical analysis of the cartilage IHC staining in the sham group and UAC-induced OA rats with si-NC or si-FSTL1 injected group. FSTL1 indicates follistatin-like protein 1; HE, hematoxylin-eosin; IHC, immunohistochemical; OA, osteoarthritis; UAC, unilateral anterior crossbite.

Article Snippet: FSTL1 concentration in synovium fluid was measured by Human FSTL1 ELISA Kit (Cusabio) according to the kit instructions.

Techniques: Staining, Injection, Immunohistochemistry, Immunohistochemical staining

Journal: The Journal of Pathology

Article Title: Dynamic change of polarity in spread through air spaces of pulmonary malignancies

doi: 10.1002/path.6382

Figure Lengend Snippet: TGF‐β1‐induced FSTL1 secretion from 2D‐MAOs affects the attachment of CRC organoids. (A) Heatmap of 2D‐MAO microarray analysis. Triplicates for each condition (TGF‐β1‐treated, Tgf; non‐treated, Ctrl) are shown. (B) Venn diagram of differentially expressed genes (DEGs) upregulated in TGF‐β1‐treated 2D‐MAOs and listed genes in the database for apical membrane proteins in human airway epithelial cells. Four common genes are shown. (C) Western blotting for FSTL1 in 2D‐MAOs treated with TGF‐β1. (D) Immunostaining of FSTL1 for TGF‐β1‐treated 2D‐MAOs. Green, FSTL1; red, phalloidin; blue, Hoechst 33342. Scale bar: 20 μm (E) Adhesion rate of C45 organoids to 2D‐MAOs. The indicated doses of rhFSTL1 were added to the co‐culture medium. (F) Adhesion rate of C45 organoids to 2D‐MAOs. Either C45 (Org) or 2D‐MAOs (MAO) were pre‐treated with TGF‐β1 before the assay. (G) Ratio of polarity status of rhFSTL1‐treated (rhFSTL1) and non‐treated (Ctrl) organoids. (H–J) Quantitative analysis of the no green fluorescence at the organoid surface (NFOS) ratio (H), adhesion rate (I), and detachment rate (J) for rhFSTL1‐treated (rhFSTL1) and non‐treated (Ctrl) organoids. (K) Western blotting of FSFL1 infected with two different shRNAs against FSTL1 (#1 and #2) and shRNA with scrambled sequence (Scr). (L–N) Quantitative analysis of NFOS ratio (L), adhesion rate (M), and detachment rate (N) for shFSTL1‐infected (#1, #2) and scramble shRNA‐infected (Scr) organoids. Organoids were treated with TGF‐β1 (K–N). ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The assays using cytokines, dasatinib, and recombinant human FSTL1 (rhFSTL1; Peprotech, Cranbury, NJ, USA) are illustrated in supplementary material, Figure .

Techniques: Microarray, Membrane, Western Blot, Immunostaining, Co-Culture Assay, Fluorescence, Infection, shRNA, Sequencing

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 1. Effect of high glucose induction on FSTL1 expression in HK-2 cells. (A, B) Effect of high glucose concentration on FSTL1 expression in HK-2 cells. (A) FSTL1 protein expression (Western blotting); (B) FSTL1 mRNA expression (qPCR). (C, D) Effect of high glucose duration of action on FSTL1 expression in HK-2 cells. (C) FSTL1 protein expression (Western blotting); (D) FSTL1 mRNA expression (qPCR). (E, F) Effect of osmotic pressure on FSTL1 expression in HK-2 cells. (E) FSTL1 protein expression (Western blotting); (F) FSTL1 mRNA expression (qPCR). *P < 0.05 and **P < 0.01. NG, normal glucose; HG, high glucose; Mannitol, high osmolarity.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Expressing, Concentration Assay, Western Blot

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 2. Effect of FSTL1 on proliferation of high glucose-induced HK-2 cells. (A, B) CCK-8 assays for cell proliferation. (C, D) Flow cytometry assays for cell proliferation. (A, C) Silencing FSTL1 expression; (B, D) Giving rhFSTL1. Compared to the NG group, *P < 0.05 and **P < 0.01. Compared to the HG group, #P < 0.05 and ##P < 0.01. shFSTL1, silencing of FSTL1; rhFSTL1, recombinant human FSTL1.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: CCK-8 Assay, Flow Cytometry, Expressing, Recombinant

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 3. Effect of FSTL1 on oxidative stress in high glucose-induced HK-2 cells. (A, B, C) Silencing of FSTL1 by shRNA attenuated HG-induced oxidative stress. (A) GSH level; (B) SOD level; (C) MDA level. (D, E, F) rhFSTL1 aggravated HG-induced oxidative stress. (D) GSH level; (E) SOD level; (F) MDA level. Compared to the NG group, **P < 0.01. Compared to the HG group, #P < 0.05 and ##P < 0.01. GSH, glutathione; SOD, superoxide dismutase; MDA, malondialdehyde.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: shRNA

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 4. Effect of FSTL1 on transdifferentiation of high glucose-induced HK-2 cells. (A) Silencing FSTL1 expression; (B) Giving rhFSTL1. Compared to the NG group, *P < 0.05 and **P < 0.01. Compared to the HG group, #P < 0.05 and ##P < 0.01.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Expressing

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 5. Effect of FSTL1 on cell migration of high glucose-induced HK-2 cells. Compared to the NG group, **P < 0.01. Compared to the HG group, #P < 0.05 and ##P < 0.01.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Migration

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 6. Effect of FSTL1 on NF-κB expression in high glucose-induced HK-2 cells. (A) Silencing FSTL1 expression; (B) Giving rhFSTL1. Compared to the NG group, *P < 0.05 and **P < 0.01. Compared to the HG group, #P < 0.05 and ##P < 0.01.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Expressing

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 7. Effect of FSTL1 on HK-2 cell proliferation after blocking the NF-κB signaling pathway. (A, C) CCK- 8 assays for cell proliferation. (B, D) Flow cytometry assays for cell proliferation. Compared to the LV- shFSTL1 + HG group, *P < 0.05. Compared to the rhFSTL1 + HG group, #P < 0.05. PDTC, NF-κB signaling pathway inhibitor.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Blocking Assay, CCK-8 Assay, Flow Cytometry

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 8. Effect of FSTL1 on oxidative stress in HK-2 cells after blocking the NF-κB signaling pathway. (A, B, C) Silencing FSTL1 expression. (A) GSH level; (B) SOD level; (C) MDA level. (D, E, F) Giving rhFSTL1. (D) GSH level; (E) SOD level; (F) MDA level. Compared to the LV-shFSTL1 + HG group, *P < 0.05 and **P < 0.01. Compared to the rhFSTL1 + HG group, ##P < 0.01.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Blocking Assay, Expressing

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 9. Effect of FSTL1 on transdifferentiation in HK-2 cells after blocking the NF-κB signaling pathway. (A) Silencing FSTL1 expression; (B) Giving rhFSTL1. E-cadherin, FN, and α-SMA expression levels were examined by Western blotting. Compared to the LV-shFSTL1 + HG group, *P < 0.05 and **P < 0.01. Compared to the rhFSTL1 + HG group, #P < 0.05 and ##P < 0.01.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Blocking Assay, Expressing, Western Blot

Journal: Scientific reports

Article Title: FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells.

doi: 10.1038/s41598-024-84462-5

Figure Lengend Snippet: Fig. 10. Effect of FSTL1 on cell migration in HK-2 cells after blocking the NF-κB signaling pathway. (A) Silencing FSTL1 expression; (B) Giving rhFSTL1. Compared to the LV-shFSTL1 + HG group, **P < 0.01. Compared to the rhFSTL1 + HG group, #P < 0.05.

Article Snippet: When the degree of cell fusion was 70%, the culture medium was changed to high-glucose DMEM medium containing the recombinant human FSTL1 (1694-FN-050, R&D Systems, America) at a concentration of 300 ng/ml, and incubation was continued at 37°C for 48 h.

Techniques: Migration, Blocking Assay, Expressing